Oct 21st 2019
Oct 21st 2019
In the Heck-lab at Utrecht University (www.hecklab.com) a variety of exciting research projects seek motivated researchers to tackle them. The research emphasis is on the development of advanced mass spectrometry-based methods to address questions in proteomics and structural biology. The Heck-lab houses and excellent infrastructure, including a dozen state-of-the-art mass spectrometers, advanced separation technologies, bioinformatics and laboratories for cell culture, biochemistry and molecular biology. The research group is quite vibrant with over 15 nationalities being present. Utrecht is a great place to be.
Characterization and de novo sequencing of endogenous antibodies, with the aim to pick up antibodies raised by our immune system against rheumatoid arthritis. In this project you will develop and apply methods for the profiling and in-depth characterization of the variable domain of (auto)antibodies using native, top-down and middle-down mass spectrometry approaches, leading to the personalized profiling of patient serum autoantibodies linked to rheumatoid arthritis. You have ideally a background in pharmaceutical sciences, biomedical sciences and/or analytical chemistry.
Detailed characterization and of protein-drug interactions to identify targets and undesired off-targets of drugs. Novel chemical biology approaches will be combined with affinity enrichment and mass spectrometry. Several drug molecules will be targeted, among them anti-cancer drugs. You have ideally a background in pharmaceutical sciences and/or analytical biochemistry.
You will focus on the further optimization of native mass spectrometry and top-down proteomics for the analysis of macromolecular complexes, notably viruses, with the aim to generate even higher sensitivity and resolving power, contributing to the further development of single particle charge detection mass spectrometry. As some viruses are used for gene-delivery this project will partly be in collaboration with industry. You have ideally a background in biophysical and/or analytical chemistry.
The main goal is to develop an integrated approach to characterize proteins and their assemblies in the thermophiles Pyrococcus furiosusand Thermus thermophilus. Both these organisms are evolutionary fascinating and perfectly amendable as model systems in integrated structural biology. You will be part of a consortium that will use a combination of analytical and biophysical techniques with integrative structural modelling strategies. You will focus on native MS, top-down proteomics and crosslinking mass spectrometry (XL-MS) to uncover protein complexes in an unbiased fashion, ideally in their native state and ultimately in situ. You have ideally a background in biophysical and/or analytical chemistry.